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MAXAQUIN (LOMEFLOXACIN HYDROCHLORIDE) TABLETS: SUSCEPTIBILITY TESTS

Diffusion techniques

Quantitative methods that require measurement of zone diameters give the most precise estimate of the susceptibility of bacteria to antimicrobial agents. One such standardized procedure1 that has been recommended for use with disks to test the susceptibility of organisms to Maxaquin (Lomefloxacin HCl) tablets uses the 10-mcg lomefloxacin disk. Interpretation involves correlation of the diameter obtained in the disk test with the MIC for lomefloxacin.

Reports from the laboratory giving results of the standard single-disk susceptibility test with a 10-mcg lomefloxacin disk should be interpreted according to the following criteria:

Zone Diameter (mm) Interpretation
>= 22 Susceptible(S)
19.21 Intermediate (I)
<= 18 Resistant (R)


A report of "susceptible" indicates that the pathogen is likely to be inhibited by generally achievable drug concentrations. A report of "intermediate" indicates that the result should be considered equivocal, and, if the organism is not fully susceptible to alternative clinically feasible drugs, the test should be repeated. This category provides a buffer zone that prevents small uncontrolled technical factors from causing major discrepancies in interpretation. A report of "resistant" indicates that achievable drug concentrations are unlikely to be inhibitory, and other therapy should be selected.

Standardized susceptibility test procedures require the use of laboratory control organisms. The 10-mcg lomefloxacin disk should give the following zone diameters:

Organism Zone Diameter (mm)
S aureus (ATCC 25923) 23.29
E coli (ATCC 25922) 27.33
P aeruginosa (ATCC 27853) 22.28


Dilution techniques

Use a standardized dilution method2 (broth, agar, or microdilution) or equivalent with lomefloxacin powder. The MIC values obtained should be interpreted according to the following criteria:

MIC (mcg/mL) Interpretation
<= 2 Susceptible (S)
4 Intermediate (I)
>= 8 Resistant (R)


As with standard diffusion techniques, dilution methods require the use of laboratory control organisms. Standard lomefloxacin powder should provide the following MIC values:

Organism MIC (mcg/mL)
S aureus (ATCC 29213) 0.25.2.0
E coli (ATCC 25922) 0.03.0.12
P aeruginosa (ATCC 27853) 1.0.4.0




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